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The answer depends on the question: Optimal conditions for western blot characterization of muscle collagen type 1 depends on desired isoform. - Abstract - Europe PMC
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An optimized protocol to analyze membrane protein degradation in yeast using quantitative western blot and flow cytometry - ScienceDirect
![Just out of curiosity: Does anybody have an experience of what happens to your protein samples if you overboil them in Laemli buffer (with DTT)? Just out of curiosity: Does anybody have an experience of what happens to your protein samples if you overboil them in Laemli buffer (with DTT)?](https://i1.rgstatic.net/ii/profile.image/753454855237634-1556649044777_Q512/Ajay-Ramesh.jpg)
Just out of curiosity: Does anybody have an experience of what happens to your protein samples if you overboil them in Laemli buffer (with DTT)?
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